Fluorescence Lifetime Imaging (FLIM) is an advanced microscopy technique used to measure the fluorescence lifetime of a fluorophore in a sample. The fluorescence lifetime refers to the average time a molecule remains in its excited state before emitting a photon and returning to the ground state. Unlike traditional fluorescence microscopy, which measures the intensity of emitted light, FLIM focuses on the temporal aspect of fluorescence decay, providing unique insights into the molecular environment and interactions.
