What are the Technical Challenges and Limitations?
While FLIM offers many advantages, there are also some challenges:
1. Complex Data Analysis: The extraction of accurate lifetime information requires sophisticated data analysis and fitting algorithms, which can be computationally intensive. 2. High Cost and Technical Requirements: FLIM systems are often more expensive and complex than traditional fluorescence microscopes, requiring specialized equipment and expertise. 3. Limited Depth Penetration: Like other optical microscopy techniques, FLIM has limited penetration depth in thick tissues, which can be mitigated to some extent using techniques like multiphoton excitation.
