What histological techniques are used to study MMPs?
Several histological techniques are employed to study MMPs, including immunohistochemistry (IHC), in situ hybridization (ISH), and zymography. IHC uses antibodies to detect MMP proteins in tissue sections, allowing for localization and quantification. ISH detects MMP mRNA, providing information about gene expression patterns. Zymography is a specialized technique that assesses MMP activity by separating proteins based on their ability to degrade gelatin or casein substrates.
