What Histological Techniques Are Used in Aquatic Toxicology?
Several histological techniques are employed to investigate the effects of toxins on aquatic organisms:
1. Light Microscopy: This is the most common method used to examine stained tissue sections. It allows for the observation of general tissue structure and identification of histopathological changes. 2. Electron Microscopy: This technique offers higher resolution images, enabling the examination of subcellular structures and ultrastructural changes caused by toxins. 3. Immunohistochemistry: This method uses antibodies to detect specific proteins or other molecules within tissues. It helps identify specific cellular responses to toxins. 4. Histochemical Staining: Various stains are used to highlight different tissue components, such as proteins, lipids, and carbohydrates, providing detailed information about the biochemical changes induced by toxins.
