Tissue samples from aquatic organisms are typically collected through biopsy or necropsy. Once collected, the samples undergo fixation, usually with formalin, to preserve the tissue structure. The fixed tissues are then embedded in paraffin wax, sectioned into thin slices using a microtome, and mounted onto microscope slides. These sections are stained with specific dyes to highlight different cellular components, making it easier to observe and analyze under a microscope.
