How to Preserve RNA Integrity in Histological Samples?
To preserve RNA integrity, certain best practices should be followed:
1. Use RNase-free Tools and Reagents: Ensure all equipment and reagents are free from RNase contamination. 2. Optimize Fixation Protocols: Use fixatives that preserve RNA well, such as RNAlater, and minimize fixation time. 3. Proper Storage: Store samples at low temperatures, preferably in liquid nitrogen or at -80°C. 4. Rapid Processing: Minimize the time between tissue collection and RNA extraction to reduce degradation risk.
