Sample Collection: Proper collection of tissue samples is the first critical step. Inadequate or improper collection can lead to artifacts that affect the accuracy of the results. Fixation: Proper fixation is essential to preserve tissue morphology and prevent degradation. Incorrect fixation can alter the tissue structure and lead to inaccurate interpretations. Staining Techniques: The choice and application of staining techniques are crucial. Inconsistent staining can result in poor visualization of tissue components, affecting diagnostic accuracy. Sectioning: Thin and uniform sections are necessary for accurate analysis. Uneven or thick sections can obscure details and lead to misinterpretation. Microscopy: The quality of the microscope and the expertise of the person interpreting the slides are significant factors. High-resolution imaging and expert interpretation contribute to accuracy.
