Sectioning artifacts can result from a variety of factors, including:
Improper Fixation: Poorly fixed tissues are more prone to artifacts because they are not adequately preserved, leading to tissue distortion during sectioning. Incorrect Embedding: Incorrect orientation or incomplete infiltration of embedding media can result in uneven sections that are prone to artifacts. Microtome Issues: Problems with the microtome, such as a dull blade, incorrect blade angle, or improper cutting speed, can introduce a range of artifacts. Environmental Factors: Conditions such as humidity, temperature, and air currents can affect the quality of tissue sections and contribute to artifacts.
