While it may be impossible to completely eliminate sectioning artifacts, several strategies can be employed to minimize their occurrence:
Proper Fixation: Ensuring that tissues are thoroughly fixed can significantly reduce the likelihood of artifacts. Optimize Embedding: Carefully embedding tissues in an appropriate medium and ensuring proper orientation can lead to more consistent and artifact-free sections. Maintain Sharp Blades: Regularly replacing or sharpening microtome blades can reduce the occurrence of artifacts such as chatter and knife marks. Adjust Microtome Settings: Properly calibrating the microtome, including blade angle and cutting speed, can help produce cleaner sections. Control Environmental Conditions: Maintaining a stable environment in the cutting area can help minimize artifacts due to external factors. Handle Sections Carefully: Using fine brushes or forceps to manipulate sections can prevent tears, folds, and other physical artifacts.
