What Are Common Issues and How to Troubleshoot Them?
Some common issues that may arise during deparaffinization include:
Incomplete Removal of Paraffin: If paraffin is not entirely removed, staining may be uneven or insufficient. Ensure that the tissue sections are adequately immersed in xylene and that fresh xylene is used. Tissue Damage: Prolonged exposure to xylene or ethanol can damage tissue sections. Adhere to recommended times to prevent this. Inconsistent Staining: Inadequate deparaffinization can lead to inconsistent staining. Follow the protocol carefully, ensuring all steps are performed correctly.
