1. Fixation: Tissue samples are fixed using formalin or other fixatives. 2. Deparaffinization: Paraffin-embedded sections are deparaffinized and rehydrated through graded alcohols to water. 3. Staining with Verhoeff's Solution: Sections are stained with Verhoeff's solution for a specific duration. 4. Differentiation: Sections are differentiated with ferric chloride until elastic fibers stand out clearly. 5. Counterstaining with Van Gieson: Sections are counterstained with Van Gieson solution. 6. Dehydration and Mounting: Sections are dehydrated, cleared, and mounted for microscopic examination.
