The VVG staining technique involves multiple steps: 1. Verhoeff's Stain: Tissues are first stained with Verhoeff's solution, which contains hematoxylin, ferric chloride, and iodine. This solution stains the elastic fibers dark blue to black. 2. Differentiation: Excess stain is removed using ferric chloride, which acts as a differentiator. 3. Van Gieson Counterstain: The tissue is then counterstained with Van Gieson solution, which contains picric acid and acid fuchsin. This step stains collagen fibers red and muscle fibers yellow.
