Fixation: The tissue sample is immersed in formalin for a specific period, which can range from a few hours to several days, depending on the size and type of tissue. Dehydration: The fixed tissue is then dehydrated through a series of graded alcohol baths to remove water. Clearing: The dehydrated tissue is treated with a clearing agent, such as xylene, to remove alcohol and make the tissue transparent. Embedding: The cleared tissue is infiltrated with molten paraffin wax and then allowed to solidify, forming a paraffin block. Sectioning: The paraffin block is cut into thin sections using a microtome, and the sections are placed on glass slides for staining and microscopic analysis.
