The process of Edu labelling typically involves the following steps:
Incorporation of Edu into the cells by adding it to the culture medium or administering it to the organism. Harvesting the cells or tissues at the desired time point. Fixation of the samples to preserve cellular and tissue structures. Permeabilization of the cells to allow access for the detection reagents. Click reaction with a fluorescent azide to detect Edu-incorporated DNA. Visualization and analysis using fluorescence microscopy.
