While washing protocols can vary, a typical washing protocol might include the following steps:
Initial Rinse: After fixation, rinse the tissue sections with distilled water to remove fixatives. Intermediate Wash: During staining, wash the sections with buffer solutions between the application of different stains or reagents. Final Wash: After the staining process is complete, wash the sections thoroughly with distilled water or appropriate solutions to remove excess stains. Dehydration: Wash the sections with increasing concentrations of alcohol if dehydration is required before mounting.
