Several factors contribute to technical variability in histology, including:
Fixation: The type and duration of fixation can significantly impact tissue preservation and staining quality. Sectioning: Variations in microtome settings, blade sharpness, and section thickness can lead to inconsistent tissue slices. Staining: Differences in staining protocols, reagent quality, and incubation times can affect the intensity and specificity of staining. Embedding: The choice of embedding medium and its consistency can influence tissue orientation and sectioning quality. Microscopy: Variations in microscope settings, illumination, and magnification can affect the visualization of histological features.
