To minimize cell line variability, researchers can adopt several strategies:
Use authenticated cell lines to ensure the identity and purity of the starting material. Monitor and control culture conditions consistently across experiments. Limit the number of passages to reduce the accumulation of genetic and phenotypic changes. Implement quality control measures such as regular mycoplasma testing and karyotyping. Use cryopreservation to create cell line stocks, ensuring that experiments can be repeated with cells of the same passage number.
