What are the Resolution Limits of Light Microscopy?
The resolution limit of light microscopy is primarily constrained by the diffraction of light. According to the Abbe diffraction limit, the formula for resolution is:
Resolution (d) = λ / (2 NA)
Where λ is the wavelength of light used, and NA is the numerical aperture of the lens. For example, using light with a wavelength of 500 nm and a numerical aperture of 1.4, the resolution limit would be approximately 179 nm.
