There are several methods used for lineage tracing, each with its own advantages and limitations:
Genetic Labeling Genetic labeling involves the use of reporter genes like GFP (Green Fluorescent Protein) that are inserted into the genome. These genes are then activated in specific cells, allowing researchers to trace the lineage of these cells through multiple generations.
Cre-LoxP System The Cre-LoxP system is a widely used genetic tool for lineage tracing. It involves the insertion of LoxP sites around a gene of interest, which can then be excised by the Cre recombinase enzyme. This system allows for precise control over gene expression and lineage tracing.
Barcoding Barcoding involves the introduction of unique DNA sequences into cells. These sequences act as molecular barcodes that can be used to identify and track the progeny of the labeled cells. This method is particularly useful for high-throughput analysis of large cell populations.
