What are the methods of cell counting and viability assessment?
Cell counting and viability assessment are crucial for evaluating the success of cell culture. Common methods include the trypan blue exclusion test and automated cell counters. The trypan blue exclusion test involves mixing a cell suspension with trypan blue dye and counting the dyed (dead) and undyed (live) cells using a hemocytometer. Automated cell counters, such as the Coulter Counter or flow cytometers, provide rapid and accurate cell counts and viability assessments.
