Vacuum infiltration typically involves placing the tissue samples in a chamber where a vacuum is applied. This process often follows tissue dehydration and clearing steps. The key stages include: 1. Dehydration: Removing water from the tissue using a series of alcohol solutions. 2. Clearing: Replacing the alcohol with a clearing agent such as xylene, which is miscible with both alcohol and paraffin. 3. Infiltration: Applying a vacuum to remove air and facilitate the penetration of the embedding medium (usually molten paraffin) into the tissue.
