Maintaining and subculturing cell cultures involve regular monitoring and feeding of the cells. Cells are observed under a microscope to check for confluency, morphology, and any signs of contamination. Once cells reach a certain confluency, they are subcultured to prevent overcrowding and nutrient depletion. Subculturing involves detaching cells from the culture dish using enzymatic or mechanical methods and re-plating them in fresh culture dishes with new medium.
