- Endocardium: This is the innermost layer of the heart, lining the heart chambers. It consists of endothelial cells and connective tissue. - Myocardium: The middle and thickest layer, it is composed of cardiac muscle cells or cardiomyocytes. This layer is responsible for the contractile function of the heart. - Epicardium: The outermost layer, also known as the visceral layer of the pericardium, consists of connective tissue and fat, providing a protective layer to the heart.