While the N:C ratio is a useful parameter, it has some limitations:
Subjectivity: Estimating the N:C ratio can be subjective and depends on the experience of the histologist. Variability: The ratio can vary significantly between different cell types and even within the same tissue, making it challenging to establish universal reference values. Complementary Methods: The N:C ratio should be used in conjunction with other diagnostic methods for a more comprehensive analysis.
