What are the Limitations of Using Con A in Histology?
While Con A is a powerful tool, it has some limitations:
Non-specific binding: Con A can sometimes bind to non-target molecules, leading to background staining. Limited specificity: Con A primarily binds to mannose and glucose residues, so it may not detect all types of carbohydrate modifications. Potential for tissue damage: Harsh fixation and staining conditions can sometimes damage tissues, affecting the quality of the results.
