Con A is commonly used in tissue staining protocols. It can be conjugated to various fluorophores or enzymes (e.g., horseradish peroxidase) to facilitate detection. The staining process typically involves the following steps:
Fixing the tissue to preserve its structure and components. Blocking non-specific binding sites to reduce background staining. Incubating the tissue with Con A to allow binding to specific carbohydrate residues. Washing to remove unbound Con A. Visualizing the bound Con A using appropriate detection methods (e.g., fluorescence microscopy).
