1. False Positives: Although specific, the TUNEL assay can occasionally label DNA breaks from non-apoptotic processes, such as necrosis or autolysis. Proper controls are essential to distinguish these events. 2. Technical Sensitivity: The staining can be technically challenging, requiring a balance between sensitivity and specificity to avoid over- or under-labeling. 3. Time-Consuming: The process can be labor-intensive and time-consuming, especially when optimizing for different tissue types or conditions.
