In histology, HIFs are studied using various techniques. Immunohistochemistry (IHC) allows for the localization and quantification of HIFs in tissue sections. This technique involves the use of specific antibodies to detect HIF proteins, providing insights into their spatial distribution and expression levels in different tissues under normoxic and hypoxic conditions. Additionally, in situ hybridization can be used to examine the expression of HIF target genes at the mRNA level.
