While SA β-gal staining is a popular method for detecting senescence, it does have limitations. The enzyme activity can be influenced by factors such as cell type, culture conditions, and tissue processing techniques. Additionally, SA β-gal is not exclusively specific to senescent cells, as some non-senescent cells can also exhibit β-galactosidase activity at pH 6.0. These limitations necessitate the use of complementary markers and methods for accurate senescence detection.
