What are the Limitations of Neutral Buffered Formalin?
While NBF is highly effective, it has some limitations:
Toxicity: Formaldehyde is a toxic substance and requires careful handling and appropriate safety measures. Over-fixation: Prolonged exposure can lead to over-hardening of tissues, which might make sectioning difficult. Chemical Reactions: Formaldehyde can react with some tissue components, potentially interfering with certain staining techniques.
