What are the Steps in Using Neutral Buffered Formalin?
1. Specimen Collection: Tissue samples are collected and trimmed to appropriate sizes. 2. Fixation: Samples are immersed in NBF, usually for 24-48 hours, depending on the size and type of tissue. 3. Processing: Post-fixation, tissues are processed through a series of alcohols and clearing agents before embedding. 4. Embedding: Fixed tissues are embedded in paraffin wax to facilitate sectioning. 5. Sectioning: Thin sections of tissue are cut using a microtome and mounted on slides. 6. Staining: Slides are stained to visualize different cellular components and structures under a microscope.
