What Are the Limitations of Masson's Trichrome Staining?
While Masson's Trichrome is a powerful tool, it has some limitations:
The staining process is relatively time-consuming and requires careful handling to avoid artifacts. It may not differentiate all tissue types clearly in every sample, especially in highly fibrotic tissues where collagen may mask other components. Interpretation of the results requires expertise, as color intensity can vary depending on the exact protocol and tissue type.
