Performing technical replicates in histology involves several steps:
Sample Preparation: Prepare multiple sections from the same tissue block to ensure uniformity. Staining: Apply the same staining protocol to each section to assess the consistency of the staining process. Imaging: Capture images of the stained sections using the same imaging parameters to evaluate the reproducibility of the imaging technique. Analysis: Quantify the histological features in each replicate to compare and assess variability.
