Though magnification is a powerful tool, it has its limitations:
Optical Limitations: Light microscopes are limited by the wavelength of light, which restricts their resolving power to about 200 nanometers. Sample Preparation: Proper sample preparation is essential for achieving good results. Poorly prepared samples can result in artifacts that obscure important details. Depth of Field: As magnification increases, the depth of field (the thickness of the specimen that is in focus) decreases, which can make it challenging to view thick samples.
