What Are the Limitations of Click Chemistry in Histology?
1. Copper Toxicity: In CuAAC reactions, copper can be toxic to living cells, limiting its use in live-cell imaging. 2. Accessibility: Some click reagents can be expensive or difficult to synthesize, potentially limiting their widespread application. 3. Background Signal: In some cases, non-specific binding or incomplete washing can result in background signals, complicating data interpretation.
