Initial Rinse: Rinse instruments and surfaces with water to remove gross contaminants. Application of Detergent: Apply an appropriate detergent to break down any remaining organic material. Scrubbing: Use brushes to scrub instruments and surfaces thoroughly. Rinsing: Rinse off the detergent with water. Disinfection: Apply a disinfectant to eliminate any remaining microorganisms. Drying: Allow instruments and surfaces to air dry or use sterile wipes.