Primary antibody incubation: The sample is incubated with a primary antibody specific to the target antigen. Secondary antibody incubation: A secondary antibody conjugated to HRP is applied to bind to the primary antibody. Tyramide reaction: Tyramide substrate is added, and HRP catalyzes its conversion into a reactive form that binds covalently around the target site. Signal detection: The tagged sites are visualized using fluorescent or chromogenic substrates.
