Optimizing gel conditions involves several considerations:
Gel Concentration: The concentration of the gel matrix affects the pore size. Lower concentrations are used for larger molecules, while higher concentrations are better for smaller molecules. pH: The pH of the gel and buffer system must be suitable for the stability and activity of the molecules being analyzed. Electrophoretic Conditions: Parameters like voltage, current, and running time need to be optimized to prevent overheating and ensure efficient separation.
