What are the Key Fluorochromes Used in Flow Cytometry?
A range of fluorochromes can be used in flow cytometry, each with unique excitation and emission properties. Some commonly used fluorochromes include:
- FITC (Fluorescein isothiocyanate): Excited by blue light and emits green fluorescence. - PE (Phycoerythrin): Excited by blue or green light and emits orange-red fluorescence. - APC (Allophycocyanin): Excited by red light and emits far-red fluorescence. - PerCP (Peridinin-Chlorophyll-Protein complex): Excited by blue light and emits red fluorescence.
