What Techniques are Used for High Resolution Imaging?

Several techniques are employed for high resolution imaging in histology. Some of the most notable ones include:

1. Electron Microscopy (EM): This technique uses a beam of electrons to create detailed images of tissue samples. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) are two main types of EM, each providing different types of information about the sample.
2. Confocal Microscopy: This optical imaging technique increases optical resolution and contrast by using point illumination and a spatial pinhole to eliminate out-of-focus light.
3. Super-Resolution Microscopy: Techniques such as STORM (Stochastic Optical Reconstruction Microscopy) and PALM (Photoactivated Localization Microscopy) break the diffraction limit of light microscopy, allowing for imaging at the nanometer scale.
4. Multiphoton Microscopy: This technique uses multiple photons to excite fluorophores in a way that reduces photodamage and allows imaging of deeper tissue sections.