What are the Histological Techniques to Study Cholesterol?
Several histological techniques can be employed to study cholesterol distribution and metabolism. These include:
Staining Methods: Oil Red O and Sudan IV are commonly used to stain lipids, including cholesterol esters, in tissue sections. Immunohistochemistry: Specific antibodies against cholesterol-binding proteins can be used to localize cholesterol in tissues. Electron Microscopy: Provides detailed images of cholesterol-rich structures, such as lipid droplets and myelin sheaths, at the ultrastructural level.
