A cryostat typically consists of several key components:
1. Refrigerated Chamber: Maintains the low temperature required for tissue sectioning. 2. Microtome: A precision cutting instrument housed within the refrigerated chamber, used to slice the frozen tissue. 3. Specimen Holder: Secures the tissue sample in place during sectioning. 4. Knife or Blade Holder: Holds the cutting blade, which sections the tissue. 5. Anti-Roll Plate: Prevents the tissue sections from curling after being cut, facilitating easier handling and mounting on slides.
