1. Freezing the Tissue: The tissue sample is embedded in an optimal cutting temperature (OCT) compound and rapidly frozen. 2. Mounting the Sample: The frozen tissue is mounted onto the specimen holder within the cryostat. 3. Sectioning: The microtome is adjusted to the desired thickness, and the tissue is sliced into thin sections. 4. Collecting Sections: The sections are collected onto glass slides, often with the help of a brush or anti-roll plate. 5. Staining and Analysis: The sections are then stained and analyzed under a microscope.
