While both CISH and FISH are in situ hybridization techniques, they differ primarily in the detection methods. FISH uses fluorescently labeled probes and requires a fluorescence microscope for signal detection. In contrast, CISH uses chromogenic dyes, allowing visualization with a standard bright-field microscope. FISH is generally more sensitive and can detect low-abundance targets more effectively, but CISH offers the advantage of easier interpretation and does not require specialized equipment.
