What are the Common Methods for DNA Isolation in Histology?
Several methods are employed for DNA isolation from tissue samples, including:
Phenol-Chloroform Extraction: This method involves the use of organic solvents to separate DNA from proteins and other cellular components. Silica Column-Based Purification: Utilizes silica columns to selectively bind DNA, allowing for the removal of contaminants through washing steps. Magnetic Bead-Based Purification: Involves the use of magnetic beads coated with DNA-binding molecules to isolate DNA from lysed cells. Chelex 100 Resin: A chelating resin that binds divalent cations, thereby protecting DNA from degradation during the isolation process.
