How is the End-Point of Decalcification Determined?
Determining the end-point of decalcification is critical to ensure that the tissue is adequately decalcified without overexposure to the decalcifying agent. Several methods are used to assess the completion of decalcification:
Physical Testing: Probing the tissue with a needle to check for softness. Chemical Testing: Using chemical indicators to detect the presence of calcium ions in the decalcifying solution. Radiography: X-ray imaging to verify the removal of calcium.
