Despite careful optimization, several issues can arise during staining:
Non-Specific Staining: This can be minimized by using appropriate blocking agents and optimizing antibody concentrations in IHC. Fading and Bleaching: Proper storage of stained slides and using anti-fade mounting media can preserve staining intensity. Inconsistent Staining: Ensuring uniform tissue section thickness and even reagent application can help achieve consistent results. Background Staining: Optimizing washing steps and using high-quality reagents can reduce unwanted background staining.
