optimization of staining protocols

How to Select the Appropriate Staining Technique?

The choice of staining technique depends on the specific goals of the histological examination:
Hematoxylin and Eosin (H&E) Stain: Widely used for general tissue morphology, providing a clear distinction between nuclear and cytoplasmic components.
Special Stains: Techniques like Periodic Acid-Schiff (PAS) and Masson's Trichrome are used for specific tissue components like carbohydrates and connective tissue.
Immunohistochemistry (IHC): Utilizes antibodies to detect specific antigens, offering high specificity for proteins of interest.
In Situ Hybridization (ISH): Allows detection of nucleic acids within tissue sections, useful for studying gene expression.

Frequently asked queries:

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