Fixation: Tissue samples are fixed, typically in formalin, to preserve their structure. Dehydration: Samples are dehydrated through a series of alcohol washes. Embedding: Tissues are embedded in paraffin for sectioning. Staining: Sections are stained with Luxol Fast Blue solution, which binds to the myelin. Clearing: Excess stain is removed, and a counterstain may be applied for contrast. Mounting: Sections are mounted on slides for microscopic examination.
