Several methods are commonly used for detection in histology:
Staining Techniques: These include Hematoxylin and Eosin (H&E) staining, which is the most widely used method for general tissue staining. Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissues, providing both localization and quantification information. In Situ Hybridization (ISH): Used to detect specific nucleic acid sequences within the tissue sections. Fluorescence: Utilizes fluorescent dyes or proteins to label different cellular structures, which can then be visualized using a fluorescence microscope.